CLEMSON UNIVERSITY
 LARSEN LABORATORY DRUG DELIVERY - BIOMATERIALS - NANOTECHNOLOGY COLLEGE OF ENGINEERING, COMPUTING, AND APPLIED SCIENCE
CHEMICAL AND BIOMOLECULAR ENGINEERING
CONTACT email larsenj at clemson . edu phone 864.656.2621 office 130 Earle Hall
Jessica Tetterton
MODULATION OF NERVE REGENERATION THROUGH POLYMERSOME DELIVERY OF PEPTIDES funding sources: Creative Inquiry Collaborators: Jeff Twiss, Professor and Chair of Biological Sciences, University of South Carolina Nerve cell damage is repaired within the Peripheral Nervous System (PNS) by translation of mRNA using neurotrophic factors. However, this translation isn’t successful within the Central Nervous System (CNS). Specifically, RasGAP SH3 domain binding protein 1 (G3BP1) forms stress granules, preventing nerve regeneration. To breakdown stress granules, peptides from different domains of G3BP1 have been developed, with one peptide able to limit stress granule formation. However, the blood-brain and nerve-blood barriers prevent small molecules and drugs from entering the brain and nervous system, which limits the effectiveness of free peptide injections. Nanoparticles made from amphiphilic di-block copolymers can protect and deliver the peptides past these barriers using Apolipoprotein E (ApoE), which targets the blood-brain barrier. This can improve CNS delivery and help nerve regeneration. Additionally, by understanding the cellular uptake methods and intracellular degradation employed by polymersomes, nanoparticle properties can be fine tuned to improve targeted delivery. Valerie Zawrotuk
related presentations 1. Brady, C.; Evers, A.; Sahoo, P.; Twiss, J.; Larsen, J. “Optimization of Polymersomes for Delivery of Nerve-Regenerating Peptides.” Biomedical Engineering Society Annual Meeting. Atlanta, GA. (October 2018). 2. Brady, C.; Evers, A.; Sahoo, P.; Twiss, J.; Larsen, J. “Enhanced Cellular Delivery of Polymersomes with Encapsulated Neuron-Regenerating Peptides.” Biomedical Engineering Society Annual Meeting. Philadelphia, PA. (October 2019).